1. Introduction to the NGC Chromatographic System

2. Protein purification systems – yesterday & today
Simplified, a chromatography system is just:
Pumps
Valves
(Columns)
Detector(s)
Fraction Collector

3. NGC System Capabilities
Technical Overview
Open platform compatible with all medium pressure chromatography columns
10 ml/min or 100 ml/min system pumps
Buffer inlet module and buffer blending module for scouting and automated buffer preparation
Flexible sample loading options via sample loop (static or dynamic) and a dedicated sample pump all without the need to re-plumb the system, a C96 autosampler can also be connected to the inject valve
Column switching module enables up to 5 columns be connected. Contains an internal bypass function for system priming, and reverse flow functionality for affinity chromatography elution and column cleaning
Single wavelength detector (255/280nm), or multi-wavelength detector enabling the simultaneous measurement of 4 wavelengths (UV & Vis). Both detectors include an integrated conductivity monitor.
pH module with integrated calibration port and bypass port
Flexible fraction collection options from 96 well plates, to a range of tubes and large volume bottles
Bulletins 6286, 6326, and 6608

4. A chromatography platform that aligns to your needs
Quest™ & Quest™ Plus
Workhorse system for
the purification of proteins
peptides and nucleic acids
Scout™ & Scout™ Plus
All purpose purification
system with buffer scouting
Discover ™
Versatile automated purfication
system for advanced method
development
Yes, it does look similar to GE

5. Expandable system that grows with you
Get what you need today with a single, scalable
platform that grows and adapts to your throughput
and purification needs over time
Tier Expansion

6. Plug-n-Play Modular Design
A truly modular design
Plug-n-play modules can be easily moved within the system
New modules can be added to expand system capabilities as your purification needs change
Plug-n-Play Modules
Note system must be switched off before
modules are removed

7. ChromLab Software Highlights
Touchscreen
Intuitive
Method Templates
Column Library
Scouting
Multi-D
Import GE Unicorn SW and Bio-Rad DuoFlow files
Analysis made easy
One click peak integration
Easy overlay
Fraction heat map
Concentration determination
User Management Edition and Security Edition
FREE
Bulletin 6675

8. The Heart of the System - Pumps
Two versions available, 10 and 100 mL/min
Buffer blending module allows for double flow and simplified buffer preparation
ANYONE INTERESTED IN 100 ML/MIN PUMPS IS LIKELY INTERESTED IN BBV – 180 ML/MIN IS PREFERRED OVER 100 ML/MIN BECAUSE YOU ARE NOT REDLINING THE PUMPS – SAME FOR 18 ML/MIN ON 10 ML/MIN PUMPS

9. Inlets – Great for Automation
A buffer inlet valve provides automated buffer switching.

10. Sample Loading – Volume is Key
Volumes >10 ml use a sample loading pump with air sensor.
Smaller volumes should be done with loops.

11. Column Switching Valve
Allows reverse flow
CSV can also serve as a “loop multiplier” allowing for up to 5 sample loops

12. Detectors – The “eyes” of the system
Single wavelength for labs doing simple protein OR DNA purification
Multi-Wavelength is great for labs that:
Purify Protein/DNA complexes
Purify a fluorescently labeled protein
Purify a protein with no signal at 280nm
Human Eyes
Superman’s Eyes

13. Outlet – Large Volume Fractionation
Useful for labs doing large volume work
Able to save the “flow through” in one large container rather using the BioFrac

14. NGCTM system capabilities
System Pump
10 ml/min, 25.2 MPa
100 ml/min, 10 MPa
Single-WL Detector
255/280nm
BioFracTM Fraction Collector
Unlimited Options
180 x 12mm tubes
168 x 1.5ml tubes
120 x 15mm tubes
24 x 30mm tubes
4 x 96, 48-, 24- or 12-position micro-plates
20 x unlimited vol. adaptor
Outlet valve
Sample Inj. Valve
No re-plumbing
Column Valve
Up to 5 col.
Int. Bypass
Reverse Flow
Pre- & Delta Col. Pressure
Conductivity Monitor
0.01 – 999 mS/cm
Sample Pump
Ideal for Affinity
Integrated
100 ml/min, 10 MPa
Int. Press. Sensor
Buffer Inlet Valve (A/B)
Up to 8 Inlets each
Multi-WL Detector
nm (UV/Vis)
Up to 4 simultaneous
2, 5 & 10 mm Path lengths
Buffer Blending Valve
pH Scouting
Doubles pump flow with gradient
Air Sensor
Protects Column
Up to 8 Inlets (7 Buffers, 1 Sample)
pH Probe & Valve
In situ calibration
Integrated bypass

15. Standout features Point-to-Plumb™ Pay for only what you need
Infinitely configurable
Tier expansion
Columns mount anywhere
Modules can go anywhere
Free software—for life!
Ten-second module swapping
Highly intuitive software