1. Metabolic Regulation: A Master Role for Ribulose-1,5-Bisphosphate in One-Carbon Assimilation 
Françoise Bringel, Stéphane Vuilleumier 
Current Biology 
Volume 27, Issue 20, Pages R1127-R1129 (October 2017)
DOI: /j.cub
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2. Figure 1
Model for RuBP as a master regulatory metabolite of C1 assimilation
Ribulose-1,5-bisphosphate (RuBP, yellow box) is a product of phosphoribulokinase (Prk). Prk is essential for growth with one-carbon compounds supplied as the sole source of carbon in: A) serine-cycle methylotrophs growing with reduced one-carbon compounds such as methanol (CH3OH); and (B) autotrophs fixing carbon dioxide via the Calvin–Benson-Bassham (CBB) cycle with Prk and ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) [20]. RubisCO is absent in most serine-cycle methylotrophs including M. extorquens [8]. Binding of the coinducer RuBP to a conserved effector pocket of the LysR-type transcriptional regulator QscR may funnel carbon flux into carbon assimilation, analogously to what was previously described for CbbR [15]. Transcriptional control of genes for assimilation of reduced one-carbon compounds by QscR [8,10] and of CO2 fixation by CbbR [15] are indicated by blue and green arrows, respectively. ECMP, ethyl malonyl-CoA pathway; G3P, glyceraldehyde-3-phosphate; H4F, tetrahydrofolate; 3PG, 3-phosphoglycerate; Ru5P, ribulose-5-phosphate; TCA, tricarboxylic acid.
Current Biology  , R1127-R1129DOI: ( /j.cub )
Copyright © 2017 Elsevier Ltd Terms and Conditions