1. ILK knockdown decreases mTOR signaling in PKD kidneys.
ILK knockdown decreases mTOR signaling in PKD kidneys. Representative sections from (A) WT, (B) Ilk+/+;Pkd1fl/fl;Pkhd1-Cre (Ilk+/+ PKD), (C) Ilkfl/+;Pkd1fl/fl;Pkhd1-Cre (Ilk+/−PKD), and (D) Ilkfl/fl;Pkd1fl/fl;Pkhd1-Cre (Ilk−/− PKD) mice were stained with an antibody to P-S6 (red). Tissues were also stained for DBA (green) to detect CD and the DAPI nuclear stain (blue). Arrows indicate cytoplasmic P-S6 in CD cells. All images were taken at the same magnification. Scale bar=5 µm. (E) Percentage of cytosolic P-S6–positive cells, normalized to the total nuclei in DBA-positive CDs. Data are mean±SEM for kidneys from three mice per group. **P<0.01 compared with WT, ##P<0.01 compared with Ilk+/+ PKD.
Archana Raman et al. JASN 2017;28:
©2017 by American Society of Nephrology