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Examination of Regional Lymph Nodes by Sentinel Node Biopsy and Molecular Analysis Provides New Staging Facilities in Primary Cutaneous Melanoma  Hans-Juergen.

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Presentation on theme: "Examination of Regional Lymph Nodes by Sentinel Node Biopsy and Molecular Analysis Provides New Staging Facilities in Primary Cutaneous Melanoma  Hans-Juergen."— Presentation transcript:

1 Examination of Regional Lymph Nodes by Sentinel Node Biopsy and Molecular Analysis Provides New Staging Facilities in Primary Cutaneous Melanoma  Hans-Juergen Blaheta, Ulf Ellwanger, Birgit Schittek, Evelyn Maczey, Helmut Breuninger, Gernot Rassner, Claus Garbe  Journal of Investigative Dermatology  Volume 114, Issue 4, Pages (April 2000) DOI: /j x Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

2 Figure 1 Reverse transcription–PCR products were analyzed on an ethidium bromide stained agarose gel (2%). Detection of tyrosinase mRNA identifies a 207 bp band in two lymph nodes negative by histopathology (lanes 3, 8). Lanes 1–10, lymph node samples; lane 11, positive control (SKMel-28); lanes 12–14, negative controls (for cDNA synthesis, for first, and for nested reverse transcription–PCR); lane M, 100 bp ladder marker. Journal of Investigative Dermatology  , DOI: ( /j x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions

3 Figure 2 Probability of recurrence-free survival. Probability of recurrence-free survival for patients whose SLN were histopathologically and reverse transcription–PCR positive (Histo pos., PCR pos.), for patients with histopathologically negative SLN who were reclassified by positive reverse transcription–PCR (Histo neg., PCR pos.), and for patients with SLN negative by both techniques (Histo neg., PCR neg.). Journal of Investigative Dermatology  , DOI: ( /j x) Copyright © 2000 The Society for Investigative Dermatology, Inc Terms and Conditions


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