1. Figure S3. Zhang et al. A B
Myc-SIRT1 - +
sir2a+/+
sir2a-/-
Empty vector + -
IB anti-SIR1(10E4)
sir2a
IB anti-SIRT1(10E4)
SIRT1
IB anti-b-actin
b-actin
IB anti-b-actin
b-actin
Figure S3. Characterization of a SIRT1 monoclonal antibody, 10E4, in Western blotting analyses. (A) 293T cells were transfected with either empty vector or Myc-SIRT1, thirty six hours after transfection, cells were lysed and anti-SIRT1 Western blotting analysis was performed by anti-SIRT1 antibody, 10E4. The blot was also probed with anti-b-actin antibody to ensure the protein loading. (B)sir2a+/+ and sir2a-/- MEFs were lysed and Western blotting analyses were performed as (A).