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Hair Follicles from Alopecia Areata Patients Exhibit Alterations in Immune Privilege- Associated Gene Expression in Advance of Hair Loss  Hoon Kang, Wen-Yu.

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Presentation on theme: "Hair Follicles from Alopecia Areata Patients Exhibit Alterations in Immune Privilege- Associated Gene Expression in Advance of Hair Loss  Hoon Kang, Wen-Yu."— Presentation transcript:

1 Hair Follicles from Alopecia Areata Patients Exhibit Alterations in Immune Privilege- Associated Gene Expression in Advance of Hair Loss  Hoon Kang, Wen-Yu Wu, Blanche K.K. Lo, Mei Yu, Gigi Leung, Jerry Shapiro, Kevin J. McElwee  Journal of Investigative Dermatology  Volume 130, Issue 11, Pages (November 2010) DOI: /jid Copyright © 2010 The Society for Investigative Dermatology, Inc Terms and Conditions

2 Figure 1 Differential expression of immune privilege-associated genes in the lesional margin of alopecia areata (AA)-affected patches and the perilesional scalp hair follicles as compared with normal scalp specimens. Quantitative PCR (qPCR) revealed apparent marked increased expression of IL1 receptor antagonist (IL1Ra), CD80, and macrophage inhibitory factor (MIF) in lesional margin hair follicle bulbs. Red/IK cytokine was significantly downregulated in the lesional margin. The fold change of gene expression was analyzed by qPCR and was calculated using the formula 2-ΔΔCt, using perilesional and lesional hair follicles from four AA patients versus normal hair follicles from four controls with ≥20 hair follicles per biopsy, and qPCR analysis was conducted in triplicate. Error bars represent the range factor difference (2-ΔΔCt±ΔCt standard deviation). *Indicates P<0.05 by Student’s t-test. The y axis is in log10 scale. Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2010 The Society for Investigative Dermatology, Inc Terms and Conditions

3 Figure 2 Expression of red/IK in normal and alopecia areata (AA)-affected hair follicles. Immunohistological analysis showed normal hair follicles to express red/IK (brown color) in the outer root sheath of hair follicles and to a lesser extent in the hair matrix (a, c, d). Eccrine glands also expressed some red/IK (b). Some dermal papilla cells located in more central areas of the dermal papilla structure (inset c) expressed red/IK (c). Hair follicles in the perilesional AA scalp (c, d) and lesional AA margin (e) of AA patients presented with similar red/IK expression distribution. Parallel processing of tissues using the same concentrations and incubation time periods yielded little to no chromogen deposition in perileisonal AA (g) and lesional margin (h) hair follicles compared with normal hair follicles (f). Expression in sebaceous glands and non-follicular epithelium was largely non-specific. Bar=200μm (a), 40μm (b, c), and 100μm (d–h). Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2010 The Society for Investigative Dermatology, Inc Terms and Conditions

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