1. Luteinizing hormone depletes ascorbic acid in preovulatory follicles
Michael M Guarnaccia, M.D., M Takami, M.D., Ervin E Jones, Ph.D., M.D., Sandra L Preston, M.S., Harold R Behrman, Ph.D. 
Fertility and Sterility 
Volume 74, Issue 5, Pages (November 2000)
DOI: /S (00)

2. Figure 1
Depletion of ascorbic acid in ovaries of preovulatory rats. LH or saline was injected at 10:00 a.m. into rats and the ovaries removed at the indicated times for analysis of ascorbic acid content (see Materials and Methods). The 0-h control group represents saline-injected animals from which ovaries were removed 1 h after injection. Mean ± SEM (n = 5 animals per group). ∗P<.05.
Guamaccia. LH depletes follicle ascorbic acid. Fertil Steril 2000.
Fertility and Sterility  , DOI: ( /S (00) )

3. Figure 2
Depletion of ascorbic acid in preovulatory follicles. LH or saline was injected at 10:00 a.m. into rats, the ovaries were removed, and the preovulatory follicles dissected on an ice-cold microscope stage, pooled, and frozen for later analysis of ascorbic acid (see Materials and Methods). The 0-h control group represents saline-treated rats killed immediately after injection. ∗P<.05 (vs. 0 h).
Guamaccia. LH depletes follicle ascorbic acid. Fertil Steril 2000.
Fertility and Sterility  , DOI: ( /S (00) )

4. Figure 3
Depletion of ascorbic acid in isolated follicles by LH and menadione. Preovulatory follicles were isolated and incubated with either hCG (20 IU/mL) or menadione (mendione; 100 μM) for 3 h (see Material and Methods). Follicles were removed and the ascorbic acid content determined. ∗P<.05 (vs. control).
Guamaccia. LH depletes follicle ascorbic acid. Fertil Steril 2000.
Fertility and Sterility  , DOI: ( /S (00) )

5. Figure 4
Oocyte ascorbic acid accumulation by cumulus-enclosed and denuded oocytes. Denuded oocytes (DO) or cumulus-enclosed oocytes (CEO) were isolated and incubated in the absence (open bars) or presence (solid bars) of ascorbic acid (150 μM) for 3 h, and the oocytes were subsequently isolated from OCC under ice-cold conditions before analysis by HPLC (see Materials and Methods). Mean ± SEM of 3 experiments. ND = not detectable.
Guamaccia. LH depletes follicle ascorbic acid. Fertil Steril 2000.
Fertility and Sterility  , DOI: ( /S (00) )