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Blockade of monocyte chemoattractant protein-1 by adenoviral gene transfer inhibits experimental vein graft neointimal formation  Hideki Tatewaki, MD,

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Presentation on theme: "Blockade of monocyte chemoattractant protein-1 by adenoviral gene transfer inhibits experimental vein graft neointimal formation  Hideki Tatewaki, MD,"— Presentation transcript:

1 Blockade of monocyte chemoattractant protein-1 by adenoviral gene transfer inhibits experimental vein graft neointimal formation  Hideki Tatewaki, MD, PhD, Kensuke Egashira, MD, PhD, Satoshi Kimura, MD, Takahiro Nishida, MD, PhD, Shigeki Morita, MD, PhD, Ryuji Tominaga, MD, PhD  Journal of Vascular Surgery  Volume 45, Issue 6, Pages (June 2007) DOI: /j.jvs Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

2 Fig 1 Efficacy of adenoviral gene transfer into canine interposed vein grafts shown in photomicrographs of cross-sections of vein grafts stained with X-gal 7 days after implantation in (A) untreated group and (B) Ad-lacZ-transfected group (n = 5 each). Blue cells are β-galactosidase–positive cells, and the nuclei are counterstained with hematoxylin. IM, Intima plus media; A, adventitia. Bar = 50 μm. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

3 Fig 2 Effects of adenovirus-mediated 7ND gene transfer on inflammatory and proliferative changes. A, Photomicrographs of cross-sections of vein grafts 7 days after implantation untreated and transfected with LacZ or 7ND stained immunohistochemically for monocyte/macrophage (AM-3K) and proliferating cell nuclear antigens (PCNA). IM, Intima plus media; A, adventitia. Bar = 100 μm. B, Effect of 7ND gene transfer on inflammation and (C) proliferation 7days and 28 days after implantation in control (open bars), LacZ-transfected (shaded bars), and 7ND-transfected (black bars) grafts (n = 4 each). *P < .05 vs untreated control group. Range bars reflect the standard error. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

4 Fig 3 Effects of adenovirus-mediated 7ND gene transfer on neointimal formation. A, Photographs of whole vessels of vein grafts 28 days after implantation untreated and transfected with LacZ or 7ND stained with elastica van Gieson stain at low power field. Internal layers are highlighted with white line. Bar = 1 mm. B, Photographs of cross-sections of vein grafts 28 days after implantation untreated and transfected with LacZ or 7ND stained with elastica van Gieson stain. Internal and external elastic layers are highlighted with white and blue lines, respectively. I, Intima; M, media; A, adventitia. Bar = 100 μm. C, Effect of 7ND gene transfer on neointimal area and % lumen stenosis assessed by the formula [1– (luminal area/area encroached by the internal elastic lamina) × 100] 28 days after implantation (n = 4 each). *P < .05 vs untreated control group. Range bars reflect the standard error. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

5 Fig 4 Effects of adenovirus-mediated 7ND gene transfer on endothelial regeneration. A, Photomicrographs of cross-sections of vein grafts at 3, 7, and 28 days after implantation transfected with LacZ or 7ND stained immunohistochemically with von Willebrand Factor (vWF). Bar = 100 μm. B, The percentage of endothelial recovery was assessed in LacZ-transfected and 7ND-transfected vein grafts (n = 4 each) by the formula [100 × (the length of vWF-positive layer/the length of luminal surface in cross-sections)]. NS, No significant difference. Range bars reflect the standard error. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

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