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Elafin, a Secretory Protein, is Cross-Linked into the Cornified Cell Envelopes from the Inside of Psoriatic Keratinocytes  Hiroshi Nakane, Akemi Ishida-Yamamoto,

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Presentation on theme: "Elafin, a Secretory Protein, is Cross-Linked into the Cornified Cell Envelopes from the Inside of Psoriatic Keratinocytes  Hiroshi Nakane, Akemi Ishida-Yamamoto,"— Presentation transcript:

1 Elafin, a Secretory Protein, is Cross-Linked into the Cornified Cell Envelopes from the Inside of Psoriatic Keratinocytes  Hiroshi Nakane, Akemi Ishida-Yamamoto, Dr., Hidetoshi Takahashi, Hajime Iizuka  Journal of Investigative Dermatology  Volume 119, Issue 1, Pages (July 2002) DOI: /j x Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

2 Figure 1 Immunoblot shows specificity of elafin anti-serum. A positive band of about 6 kDa is detected (arrow). Human elafin (0.2 µg) was loaded on 15% sodium dodecyl sulfate–polyacrylamide gels and immunoblotted with anti-elafin anti-serum. The sized of molecular weight standards are shown on the left. Journal of Investigative Dermatology  , 50-55DOI: ( /j x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

3 Figure 2 Elafin and involucrin were expressed in the psoriatic epidermis.(a) Double immunofluorescent microscopy of elafin and involucrin. Involucrin (red) is expressed from the lower spinous cells, whereas elafin (green) is expressed from the upper spinous cells. Nuclei are stained with 4′,6-diamidino-2-phenylindole dihydrochloride (blue). (b) Immunoperoxidase staining of elafin. Cytoplasmic elafin staining is stronger in the apical half than in the basal half in the spinous cells. It is associated with cell membrane in the cells just under the stratum corneum. Scale bar: 50 µm. Journal of Investigative Dermatology  , 50-55DOI: ( /j x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

4 Figure 3 Elafin molecules in the Golgi apparatus and in the secretory (lamellar) granules.(a) Golgi apparatus; (b, c) secretory (lamellar) granules. Post-embedding immunoelectron microscopy of the lower spinous layer. Lowicryl K11 M samples (a,b) and Lowicryl HM20 sample (c). Laminated structures of lamellar granules are appreciated in the latter. d, desmosome. N, nucleus. Scale bar: 100 nm. Journal of Investigative Dermatology  , 50-55DOI: ( /j x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

5 Figure 4 Elafin molecules are detected along the CE. (a) Large arrowheads denote elafin-positive granules that have migrated toward the cell periphery in the upper spinous cell layer. Some are accumulated at the apical side of the cells (arrows) to be released extracellularly. Some labels are also seen along the CE (small arrowheads) in the upper cell. Note that the cell membrane (*) of the lower cell without CE is undulated compared with that of the upper cell with CE. (b) Elafin labels are detected along the CE (arrows) as well as intracellular and extracellular (*) spaces in the cornified layer. d, desmosome. Scale bar: 100 nm. Journal of Investigative Dermatology  , 50-55DOI: ( /j x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

6 Figure 5 Elafin granules are seen in the cells without involucrin-positive CE, but undetectable in the cells with CE. Post-embedding double immunoelectron microscopy of involucrin (5 nm gold) and elafin (10 nm gold). (a) In the lower cell without involucrin-positive CE, elafin-containing granules (larger arrowheads) are grouped at the periphery of the cells. In the more differentiated upper cell, some involucrin labels are detected along the CE (small arrowheads). Note that the cell membrane (*) of the lower cell without CE is undulated compared with that of the upper cell with CE (arrow). d, desmosome. (b) In the cells with more matured CE, both involucrin and elafin labels are seen along the CE, but some labels are also retained within the cytoplasm. Scale bar: 100 nm. Journal of Investigative Dermatology  , 50-55DOI: ( /j x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

7 Figure 6 Elafin and involucrin are on the same side of CE. Pre-embedding immunoelectron microscopy of involucrin (10 nm gold) and elafin (5 nm gold) in a purified psoriatic CE sample. Involucrin and elafin labels are colocalized on the brushy cytoplasmic side of the CE. Scale bar: 100 nm. Journal of Investigative Dermatology  , 50-55DOI: ( /j x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

8 Figure 7 Diagram of elafin granules and CE formation.★, elafin; ♦, involucrin. Journal of Investigative Dermatology  , 50-55DOI: ( /j x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

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