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KIT Gene Deletions at the Intron 10−Exon 11 Boundary in GI Stromal Tumors  Christopher L. Corless, Laura McGreevey, Ajia Town, Arin Schroeder, Troy Bainbridge,

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1 KIT Gene Deletions at the Intron 10−Exon 11 Boundary in GI Stromal Tumors 
Christopher L. Corless, Laura McGreevey, Ajia Town, Arin Schroeder, Troy Bainbridge, Patina Harrell, Jonathan A. Fletcher, Michael C. Heinrich  The Journal of Molecular Diagnostics  Volume 6, Issue 4, Pages (November 2004) DOI: /S (10) Copyright © 2004 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

2 Figure 1 Genomic deletions that include the intron 10–exon 11 boundary. Sequence from the end of intron 10, beginning at nucleotide 75631, and the beginning of exon 11 is shown, with the deletions of all 19 cases indicated in gray. Predicted splice acceptor sites are boxed. Wild-type sequence and amino acid codons are shown at the bottom of the figure for comparison. Note that in cases 6 and 8 the deletion was accompanied by a single nucleotide substitution at the 5′ end (underlined). A separate single nucleotide polymorphism is underlined in the intron 10 sequence of case 1. The Journal of Molecular Diagnostics 2004 6, DOI: ( /S (10) ) Copyright © 2004 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

3 Figure 2 cDNA sequences. The cDNA sequences from cases 1, 5, 11, and 15 all showed the identical deletion, as predicted from the genomic analyses. The Journal of Molecular Diagnostics 2004 6, DOI: ( /S (10) ) Copyright © 2004 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions


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