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Increased expression of the CD80 accessory molecule by alveolar macrophages in asthmatic subjects and its functional involvement in allergen presentation.

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Presentation on theme: "Increased expression of the CD80 accessory molecule by alveolar macrophages in asthmatic subjects and its functional involvement in allergen presentation."— Presentation transcript:

1 Increased expression of the CD80 accessory molecule by alveolar macrophages in asthmatic subjects and its functional involvement in allergen presentation to autologous TH2 lymphocytes  Samuele E. Burastero, MDa, Zulma Magnani, PhDa, Claudio Confetti, PhDa, Laura Abbruzzese, MDa, Susanna Oddera, PhDb, Piero Balbo, MDd, Giovanni A. Rossi, MDb, Emanuele Crimi, MDc  Journal of Allergy and Clinical Immunology  Volume 103, Issue 6, Pages (June 1999) DOI: /S (99) Copyright © 1999 Mosby, Inc. Terms and Conditions

2 Fig. 1 Proliferation of freshly isolated peripheral blood and CD4-enriched T lymphocytes from patient 3 to Dermatophagoides (Der) antigen presented by autologous AMs. Results are expressed as mean values of thymidine incorporation by quadruplicate microcultures. SDs are indicated. The T cell:APC ratio was 1:5. CTLA4-Ig (a chimeric recombinant molecule binding to both CD80 and CD86) was used as a specific inhibitor of costimulation-dependent antigen presentation. CD4-Ig, an irrelevant chimeric recombinant molecule containing the same Ig domains as CTLA4-Ig, was used as a control for the specificity of CTLA4-Ig inhibition. Background proliferation was not subtracted out. CPM, Counts per minute. Journal of Allergy and Clinical Immunology  , DOI: ( /S (99) ) Copyright © 1999 Mosby, Inc. Terms and Conditions

3 Fig. 2 CD80-dependent inhibition of IL-4 and IL-5 production (A and B ) and cell proliferation (C ) by Dermatophagoides- specific CD4 TH2 lymphocytes stimulated with antigen presented by AMs from 2 asthmatic individuals (nos. 5 and 9). Monoclonal antibodies to CD80 or CD86 were used on APCs (autologous AMs) in 48-hour assays at the indicated concentrations. Isotype-matched (IgM and IgG1, respectively) mAbs of irrelevant specificity (10 μg/mL) were used as controls. Each dot represents the mean value of quadruplicate microcultures. SDs are indicated. Dotted lines indicate background values for each assay. Journal of Allergy and Clinical Immunology  , DOI: ( /S (99) ) Copyright © 1999 Mosby, Inc. Terms and Conditions

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