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Harold S. Nelson, MD, David Iklé, PhD, Andrea Buchmeier 

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Presentation on theme: "Harold S. Nelson, MD, David Iklé, PhD, Andrea Buchmeier "— Presentation transcript:

1 Studies of allergen extract stability: The effects of dilution and mixing 
Harold S. Nelson, MD, David Iklé, PhD, Andrea Buchmeier  Journal of Allergy and Clinical Immunology  Volume 98, Issue 2, Pages (August 1996) DOI: /S (96) Copyright © 1996 Mosby, Inc. Terms and Conditions

2 FIG. 1 Effect of conditions of storage on the residual potency of Bermuda grass, 10,000 AU/ml. Bermuda grass was diluted in phenol-saline-HSA (Single) or in a mixture of other allergen extracts (Mix) and stored at 4° C for 3 and 12 months. Stored and fresh dilutions were compared by inhibition ELISA. Indicated on the left are the means and pooled standard deviations for each condition of storage. On the right are the results of the Tukey-Kramer analysis. Pairs of means are not significantly different at the 0.05 level, if the corresponding comparison circles are nested or if they overlap with an outside angle of greater than 90 degrees. OD, Optical density. Journal of Allergy and Clinical Immunology  , DOI: ( /S (96) ) Copyright © 1996 Mosby, Inc. Terms and Conditions

3 FIG. 2 Effect of protease-containing extracts on timothy grass, 10,000 AU/ml. Indicated on the left are the means and pooled standard deviation for each condition of storage. Indicated on the right are the results of the Tukey-Kramer analysis. Pairs of means are not significantly different at the 0.05 level, if the corresponding comparison circles are nested or if they overlap with an outside angle of greater than 90 degrees. OD, Optical density; Alt, Alternaria tenuis; Clado, Cladosporium herbarum; CR, American cockroach; Mite, D. farinae; Pen, Penicillium notatum (all at 1:10 dilution of stock except D. farinae, which was 1:2 dilution); Single, dilution in phenol-saline-HSA alone; Mix, mixture of other allergen extracts. Journal of Allergy and Clinical Immunology  , DOI: ( /S (96) ) Copyright © 1996 Mosby, Inc. Terms and Conditions

4 FIG. 3 Effect of protease-containing extracts on short ragweed 10,000 AU/ml. Indicated on the left are the means and pooled standard deviation for each condition of storage. Indicated on the right are the results of the Tukey-Kramer analysis. Pairs of means are not significantly different at the 0.05 level, if the corresponding comparison circles are nested or if they overlap with an outside angle of greater than 90 degrees. OD, Optical density; Alt, Alternaria tenuis; Clado, Cladosporium herbarum; CR, American cockroach; Mite, D. farinae; Pen, Penicillium notatum (all at 1:10 dilution of stock except D. farinae, which was 1:2 dilution); Single, dilution in phenol-saline-HSA alone; Mix, mixture of other allergen extracts. Journal of Allergy and Clinical Immunology  , DOI: ( /S (96) ) Copyright © 1996 Mosby, Inc. Terms and Conditions


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