1. VH usage of cross-reactive B cells induced by H5N1 or H7N9 vaccination.
VH usage of cross-reactive B cells induced by H5N1 or H7N9 vaccination. (A) Flow cytometry plots showing the gate used to single-cell sort cross-reactive H5H1+ or H7H3+ B cells from H5N1 (top) or H7N9 (bottom) vaccinated donors. (B) Graphs showing the percentage of IgG+ cells that are H5 or H7 HA+ (left) and proportion of HA+ cells that also cross-react with HA from another subtype in the same group (right) in each donor as detected by flow cytometry. The red dots indicate the donors from which single-cell sorting and repertoire analysis were performed. (C and D) Comparison of percentage of Ig heavy chain sequences from all single cell–sorted H5H1+ or H7H3+ cells encoded by VH1-69, VH1-18, or VH6-1 genes, as indicated. In (C), the percentage of all sequences from each vaccine trial is shown. Differences in frequency for each of these three VH genes between vaccine studies were significant (P < , Fisher’s exact test). In (D), each dot represents the mean percentage for each donor, with the line indicating the mean for all 20 donors in each vaccine trial. P values were determined using unpaired nonparametric Mann-Whitney t test. n.s., not significant.
Sarah F. Andrews et al. Sci. Immunol. 2017;2:eaan2676
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