1. Genotoxicity assessment of mouse oocytes by comet assay before vitrification and after warming with three vitrification protocols 
Anais Berthelot-Ricou, M.D., Jeanne Perrin, M.D., Ph.D., Carole di Giorgio, Ph.D., Michel de Meo, Ph.D., Alain Botta, M.D., Pharm.D., Ph.D., Blandine Courbiere, M.D., Ph.D. 
Fertility and Sterility 
Volume 100, Issue 3, Pages (September 2013)
DOI: /j.fertnstert
Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
Alkaline comet assay on mouse oocyte after exposure to equilibration and vitrification solutions according to protocols 1, 2, and 3. Olive tail moment (OTM): product of the percentage of the DNA in the tail and the distance between the center of the head and the tail. Neg Cont: negative control oocyte group in M2 medium at ambient temperature just after hyaluronidase exposure. Pos Cont: positive control oocyte group incubated in a 250-μmol/L H2O2 solution for 5 minutes at 4°C in the dark. P1, P2, and P3: oocyte groups exposed to equilibration and vitrification solutions according to protocols 1, 2, and 3.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

3. Figure 2
Alkaline comet assay on mouse oocyte after vitrification, liquid nitrogen storage, and thawing according to protocols 1, 2, and 3. Olive tail moment (OTM): product of the percentage of the DNA in the tail and the distance between the center of the head and the tail. Neg Cont: negative control oocyte group incubated in M16 medium at 37°C and 5% CO2 for 3 hours just after hyaluronidase exposure. Pos Cont: positive control oocyte group incubated in a 250-μM H2O2 solution for 5 minutes at 4°C in the dark. P1, P2, and P3: oocytes vitrified with protocols 1, 2 and 3 and rehydrated with the corresponding thawing kits according to each manufacturer’s instructions.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions