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Development and validation of a human DNA quantification and sex determination approach based on real time PCR followed by high resolution melting analysis 

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Presentation on theme: "Development and validation of a human DNA quantification and sex determination approach based on real time PCR followed by high resolution melting analysis "— Presentation transcript:

1 Development and validation of a human DNA quantification and sex determination approach based on real time PCR followed by high resolution melting analysis  S. Ginart, E. Alechine, M. Caputo, H. Cano, D. Corach, A. Sala  Forensic Science International: Genetics Supplement Series  Volume 5, Pages e269-e271 (December 2015) DOI: /j.fsigss Copyright © 2015 Elsevier Ireland Ltd Terms and Conditions

2 Fig. 1 (a) First derivate from HRM analysis plot of control DNAs: 2800 male (in blue) and 4997A female (in pink); negative control (in red). Platform: Rotor Gene (b) Sensitivity assay: first derivate from HRM analysis plot of genomic male DNA control (Plexor® HY ADN male Standard). From top to bottom 50–10–2–0.4–0.08–0.016ng/μl dilutions; Negative control (in red). Platform: Rotor Gene 6000. Forensic Science International: Genetics Supplement Series 2015 5, e269-e271DOI: ( /j.fsigss ) Copyright © 2015 Elsevier Ireland Ltd Terms and Conditions


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