1. AKAP15 coimmunoprecipitates with CaV1
AKAP15 coimmunoprecipitates with CaV1.2 channels isolated from rat heart.
AKAP15 coimmunoprecipitates with CaV1.2 channels isolated from rat heart. (A) Amino acid sequence alignment of the LZ-like region identified in the distal C-terminal domain of CaV1.1 that interacts with AKAP15 with other members of the CaV1 family (44). Conserved leucine residues or hydrophobic residues in the ”a” and ”d” positions of the heptad repeats are indicated and asterisked. (B) Coimmunoprecipitation of AKAP15 with CaV1.2 channels isolated from rat heart extracts. Proteins were immunoprecipitated from heart extracts with an anti-CaV1.2 antibody (CNC1; lane 2) or control IgG (lane 3). Immunoblots were probed with anti-CNC1 (Upper) or anti-AKAP15 (Lower). Positive control for immunoblotting was 20 μl of heart extract (lane 1).
Joanne T. Hulme et al. PNAS 2003;100:22:
©2003 by National Academy of Sciences