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Volume 57, Issue 5, Pages (May 2000)

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1 Volume 57, Issue 5, Pages 1927-1935 (May 2000)
Estrogen worsens incipient hypertriglyceridemic glomerular injury in the obese Zucker rat  Frazier T. Stevenson, Carrie M. Wheeldon, Matthew D. Gades, George A. Kaysen, Judith S. Stern, Harry Van Goor  Kidney International  Volume 57, Issue 5, Pages (May 2000) DOI: /j x Copyright © 2000 International Society of Nephrology Terms and Conditions

2 Figure 1 Urinary albumin excretion in seven-week obese Zucker rats (OZRs). Abbreviations are: sham, sham operated; Ovx, ovariectomized; Ovx + E, ovariectomized with estrogen replacement (N = 20 to 30 per group). Kidney International  , DOI: ( /j x) Copyright © 2000 International Society of Nephrology Terms and Conditions

3 Figure 2 Total and very low density lipoprotein (VLDL) lipids in seven-week OZRs (N = 20 to 30 per group). Symbols are: (▪) sham; (□) Ovx; (▪) Ovx + E; *P < 0.05 vs. sham;+P < 0.05 vs. Ovx. Definitions are in the legend toFigure 1. Kidney International  , DOI: ( /j x) Copyright © 2000 International Society of Nephrology Terms and Conditions

4 Figure 3 Linear regression of VLDL triglycerides (TG) versus log (urinary albumin excretion, UAE) in seven-week OZRs (r2 = 0.604,P < ). Kidney International  , DOI: ( /j x) Copyright © 2000 International Society of Nephrology Terms and Conditions

5 Figure 4 Apolipoprotein scoring in seven-week OZRs. Scoring represents mean of 30 to 50 glomeruli per rat with five to six per group. Symbols are: (▪) sham; (□) Ovx; (▪) Ovx + E; *P < 0.05 vs. sham;+P < 0.05 vs. Ovx. Abbreviation ND is not detected. Kidney International  , DOI: ( /j x) Copyright © 2000 International Society of Nephrology Terms and Conditions

6 Figure 5 Representative glomerular apolipoprotein immunohistochemistry in seven-week OZRs. Sections represent staining in estrogen-supplemented (a, c, ande) and sham-operated (b, d, andf) rats. Specific antibodies against apo A-I (a and b), apo A-IV (c and d), and apo B (e and f) were used. The arrow denotes tubular apo A-IV staining (magnification ×40). Kidney International  , DOI: ( /j x) Copyright © 2000 International Society of Nephrology Terms and Conditions

7 Figure 6 Glomerular gene expression in seven-week OZRs by RT-PCR. The results were determined in duplicate (N = 4 in each group). Kidney International  , DOI: ( /j x) Copyright © 2000 International Society of Nephrology Terms and Conditions

8 Figure 7 Glomerular (A) and interstitial (B) monocyte/macrophage deposition by ED1 staining. Means represent 20 scores from four to five rats per group. Symbols are: (▪) sham; (□) Ovx; (▪) Ovx + E; *P < 0.05 vs. Ovx. Kidney International  , DOI: ( /j x) Copyright © 2000 International Society of Nephrology Terms and Conditions


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