1. Separation by HPLC of lipopeptide adducts from the tryptic digest of involucrin.
Separation by HPLC of lipopeptide adducts from the tryptic digest of involucrin. Involucrin was reacted with SLV containing lipid Z in the presence of TGase 1. Lipid-attached hydrophobic peptides were selectively retained on a C4 column under strongly desorbing solvent conditions (black line). Numbering of peaks refers to the corresponding lipopeptides in Fig. 3 and Tables 1 and 2. In controls for the TGase reaction, no lipopeptides were recovered in the presence of 5 mM EDTA (green line), and lipopeptide amounts were significantly reduced by putrescine (see Fig. 5) or by 20 mM (red line) of the generic TGase inhibitor cystamine.
Zoltán Nemes et al. PNAS 1999;96:15:
©1999 by National Academy of Sciences