1. Inhibition Zone Diameter (mm) a Gram-Negative Bacteria
table 4. The inhibition zone diameter methodological analysis of the effect of daily mouthwash (class A) on the growth of
gram-negative bacteria
Microorganis
Inhibition Zone Diameter (mm) a
control ddH20
control Pure Methanol
undiluted Disinfectant
Antiseptic DF
1/2
1/4
1/8
1/16
1/32
Ceftazidime
(30 g)
class A – Daily Mouthwash §
Fresh Burst Listerine Mouthwash (Disinfectant)
Active ingredients – Thymol (0.064%), Eucalyptol (0.092%), Methyl salicylate (0.092%), and Menthol (0.042%).
Gram-Negative Bacteria
Citrobacter koseri
NI * NI 
19.00 0.18
Enterobacter cloacae
19.00 0.15
Escherichia coli
5.33 1.57
15.00 0.15
E. coli ESBL
0.00 0.00
Klebsiella pneumoniae
25.00 0.25
Proteus vulgaris
Pseudomonas aeruginosa
14.00 0.12
Salmonella typhimurium
18.00 0.18
Shigella sonnei
a Mean value ± SEM, n = 3 (the zone of inhibition [mm] including disk of 5 mm in diameter).
* NI = No Inhibition; TI = Total Inhibition (the zone of inhibition [mm] including disk of 5 mm in diameter is > 50 mm); DF = Dilution Factor.
§ Commercial brands are disclosed in accordance with ethical and propriety issues.
Niveen M. Masri et al. The Immunomodulatory, Antimicrobial and Bactericidal Efficacy of Commonly Used Commercial
Household Disinfectants, Sterilizers and Antiseptics in Vitro: Laboratory Assessment of Anti-Inflammatory Infection
Control Mechanisms and Comparative Biochemical Analysis of the Microbial Growth of Gram-Negative Bacteria.
American Journal of Medical and Biological Research, 2015, Vol. 3, No. 1, doi: /ajmbr-3-1-1
© The Author(s) Published by Science and Education Publishing.