1. S. pneumoniae GapA is sensitive to oxygen and H2O2 exposure.
S. pneumoniae GapA is sensitive to oxygen and H2O2 exposure. (A) Specific activity of recombinant S. pneumoniae GapA at various pHs. (B) Specific activity of recombinant S. pneumoniae GapA after incubation with H2O2 (0 to 10 mM) at pH 7.0. (C) Specific activity of S. pneumoniae GapA highlighting the role of pH and H2O2 concentration. S. pneumoniae GapA is resistant to H2O2 at pH 6.0 (black circles). In the blue squares, the time-dependent inactivation of recombinant S. pneumoniae GapA is shown in the presence 0.3 mM H2O2 at pH 7.0. Green diamonds show time-dependent inactivation of recombinant S. pneumoniae GapA at pH 7.0 in the presence of 1 mM H2O2. Open symbols show the specific activity of S. pneumoniae GapA after incubation with DTT (50 mM).
John P. Lisher et al. mSphere 2017; doi: /mSphere