1. Rescue of the sel-12 Egl phenotype by PS1 and PS1ΔE9 expressed from arrays formed at a concentration of 2 μg/ml.
Rescue of the sel-12 Egl phenotype by PS1 and PS1ΔE9 expressed from arrays formed at a concentration of 2 μg/ml. Each line in the histogram represents data for an independent transgenic line; the number of hermaphrodites scored is shown above each line. The transgene is indicated on the horizontal axis. The percentage of Egl+ hermaphrodites (see Fig. 1) is indicated on the vertical axis. At 2 μg/ml of injected DNA, expression from arrays or representation of the plasmid in the arrays may be reduced, accounting for the reduced activity of SEL-12 (transgenic line 3) and PS1 (transgenic line 5) compared with arrays generated at 20 μg/ml (Fig. 1).
Diane Levitan et al. PNAS 1996;93:25:
©1996 by National Academy of Sciences