1. RNA-seq of the clk-1(qm30) (± nuclear or WT clk-1) mutants.
RNA-seq of the clk-1(qm30) (± nuclear or WT clk-1) mutants. (A) Graphical illustration of clk-1(qm30), clk-1(qm30)+nuc, and clk-1(qm30)+WT lines used for RNA-seq. mRNA was isolated from three biological replicates of each mutant, harvested at the L4 stage. (B) PCA of RNA libraries showing clear distinction between clk-1(qm30), clk-1(qm30)+nuc, and clk-1(qm30)+WT (N = 3 biological replicates for each strain). (C) Correlation matrix of RNA-seq samples shows the expected clustering of the biological triplicates of each strain. (D) Top 50 down-regulated GO terms (left graph) and up-regulated GO terms (right graph) in clk-1(qm30) versus clk-1(qm30)+WT (left column) and clk-1(qm30)+nuc versus clk-1(qm30)+WT (right column). Proportions of GO terms associated with translation and mRNA processing are depicted in yellow, development and reproduction in red, metabolism in green, and ion transport in blue.
Marte Molenaars et al. LSA 2018;1:e
© 2018 Molenaars et al.