1. Schematic illustration of polysaccharide capsule biosynthesis (A) and nucleotide/cofactor biosynthesis (B) in S. pneumoniae D39 and its relationship to glycolysis and the fate of pyruvate under aerobic conditions.
Schematic illustration of polysaccharide capsule biosynthesis (A) and nucleotide/cofactor biosynthesis (B) in S. pneumoniae D39 and its relationship to glycolysis and the fate of pyruvate under aerobic conditions. The enzymes that are found to be sulfenylated (eR ≥ 1.6, GapA) are highlighted in red, with bold red corresponding to those proteins with eR ≥ 3.0. Black indicates protein was detected in the total cell lysate, but not enriched, and gray indicates the protein was not detected in the cell lysate. Two of the three glycosyltransferases known to be involved in the synthesis of the capsule repeat unit (Cps2T and Cps2G; Cps2F is just below eR for GapA [Fig. 5C]) (65) and CpsK are all identified as sulfenylated in cells with high confidence. In panel B, key enzymes associated with synthesis and utilization of ribose-5-phosphate and sulfenylated in cells are indicated, as highlighted in panel A. Green indicates S-glutathionylated in the cell lysate (DeoB).
John P. Lisher et al. mSphere 2017; doi: /mSphere