1. Association of endogenous cadherins with CDO and BOC
Association of endogenous cadherins with CDO and BOC. (A and B) Association of CDO, cadherin, and β-catenin in C2C12 cells.
Association of endogenous cadherins with CDO and BOC. (A and B) Association of CDO, cadherin, and β-catenin in C2C12 cells. Lysates from proliferating, low-density C2C12 cells cultured in growth medium (G) or confluent cultures in differentiation medium [2% horse serum (HS)] for the indicated times were immunoprecipitated with antibodies to CDO (A) or cadherin (pancadherin antibody) (B), fractionated by SDS/PAGE, and immunoblotted with the indicated antibodies. Straight lysates were also probed as indicated. Immunoblotting for myogenin and MHC indicates the progression of cell differentiation. (C) Association of N- and M-cadherin with CDO. (D) Association of BOC, cadherin, and β-catenin in RD cells. (E) Association of cadherin and β-catenin with stably expressed BOC(flag) in C2C12 cells. (F) Association of N- and M-cadherin with stably expressed BOC(flag) in C2C12 cells. For C–F, immunoprecipitation and immunoblotting are as described for A and B.
Jong-Sun Kang et al. PNAS 2003;100:7:
©2003 by National Academy of Sciences