1. dsRBDs 3 and 4 of hStau1 dimerize on the RNA.
dsRBDs 3 and 4 of hStau1 dimerize on the RNA. (A) Size exclusion chromatography (SEC) profile of the indicated hStau1 constructs. The calculated molecular weight (MW) for a monomer is depicted on the right. The elution volume of a reference globular protein is marked by a dotted gray line, with the corresponding MW on top. Protein constructs including SSM and dsRBD5 elute as dimers. (B) SEC profiles of purified hStau1 dsRBD3-4 alone (top), or pre-incubated with ARF1 SBS (bottom). Calculated MW, obtained from MALLS, is indicated in blue. (C) EMSA of 5ʹ-fluorescein-labeled ARF1 SBS titrated with hStau1 dsRBD3-4 construct. Protein concentrations (in μM) are indicated above the gel; RNA concentration was 1 μM.
Daniela Lazzaretti et al. LSA 2018;1:e
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