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USP2a overexpression modifies the microRNA expression profile of prostate cells. USP2a overexpression modifies the microRNA expression profile of prostate.

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Presentation on theme: "USP2a overexpression modifies the microRNA expression profile of prostate cells. USP2a overexpression modifies the microRNA expression profile of prostate."— Presentation transcript:

1 USP2a overexpression modifies the microRNA expression profile of prostate cells.
USP2a overexpression modifies the microRNA expression profile of prostate cells. A, analysis of USP2a mRNA and protein expression in empty vector (Vector), USP2aMUT, and USP2aWT iPrEC transfectants. B, prostate-specific microRNA expression profiling color heat-map carried out by TaqMan quantitative reverse-transcription PCR assay in the indicated iPrEC cells. Evaluation of USP2a protein expression and microRNA level analysis performed in LNCaP cells on (C) transfection with empty vehicle (Ve), pCDNA3 (Vector), pUSP2aMUT, or pUSP2aWT; and (D) incubation with empty vehicle (Ve), control (siControl), or specific USP2a (siUSP2a) silencing oligonucleotides. All data represent mean ± standard deviation of at least three independent replicates. P values: *P < 0.05, **P < 0.01, ***P < See also Supplementary Figure S1. GAPDH, glyceraldehyde-3-phosphate dehydrogenase; WB, Western blot. Barbara Benassi et al. Cancer Discovery 2012;2: ©2012 by American Association for Cancer Research

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