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Basophil and eosinophil accumulation and mast cell degranulation in the nasal mucosa of patients with hay fever after local allergen provocation  Alex.

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Presentation on theme: "Basophil and eosinophil accumulation and mast cell degranulation in the nasal mucosa of patients with hay fever after local allergen provocation  Alex."— Presentation transcript:

1 Basophil and eosinophil accumulation and mast cell degranulation in the nasal mucosa of patients with hay fever after local allergen provocation  Alex KleinJan, BSca, Alan R. McEuen, PhDb, Mariska D. Dijkstra, MDa, Mark G. Buckley, PhDb, Andrew F. Walls, PhDb, Wytske J. Fokkens, MD, PhDa  Journal of Allergy and Clinical Immunology  Volume 106, Issue 4, Pages (October 2000) DOI: /mai Copyright © 2000 Mosby, Inc. Terms and Conditions

2 Fig. 1 Total symptom score before and after allergen provocation. After both provocations (15 and 30 minutes), symptoms including sneezing, rhinorrhea, itching, and nasal blockage were recorded. Patients were also asked to rate their symptoms on a 4-point (0-3) scale during the day after provocation after 3, 6, 9, 12, and 24 hours. Journal of Allergy and Clinical Immunology  , DOI: ( /mai ) Copyright © 2000 Mosby, Inc. Terms and Conditions

3 Fig. 2 Photomicrograph of BB1-positive cells (red) in the epithelium and in the lamina propria of a nasal mucosal biopsy section obtained from an allergic patient before (A) and 24 hours after (B) allergen provocation. For tryptase, specimens are shown from before (C) and 24 hours after (D) allergen provocation, and for chymase, specimens are shown before (E) and 24 hours after (F) allergen provocation (counterstained with Gill’s hematoxylin; magnification ×1000). Journal of Allergy and Clinical Immunology  , DOI: ( /mai ) Copyright © 2000 Mosby, Inc. Terms and Conditions

4 Fig. 3 Numbers (in cells per square millimeter) of basophils (A and B ), tryptase-positive cells (C and D ), and chymase-positive cells (E and F ) in the epithelium (A , C , and E ) and lamina propria (B , D , and F ) in nasal mucosa biopsy specimens. Nasal mucosa biopsy specimens were taken before provocation, 1 hour after provocation, 24 hours after provocation, and 1 week after provocation. Allergen challenge (arrows) was performed with 1000 and 10,000 BU grass pollen. Box plots represent the 25th-75th percentile, the range (whiskers) , and the median value. Journal of Allergy and Clinical Immunology  , DOI: ( /mai ) Copyright © 2000 Mosby, Inc. Terms and Conditions

5 Fig. 4 Mast cells. Representative sections of double staining showing tryptase (red), chymase (blue), and tryptase-chymase double-positive cells (red and blue) indicate 3 types of mast cells: tryptase-positive (MCT) , tryptase-chymase positive (MCTC) , and chymase-positive (MCC) . a, Before provocation; b, after provocation (counterstaining with methyl-green; magnification ×1000). Journal of Allergy and Clinical Immunology  , DOI: ( /mai ) Copyright © 2000 Mosby, Inc. Terms and Conditions

6 Fig. 5 The numbers (A , C , and E) and proportion (B , D , and F) of tryptase-positive mast cell (MCT) phenotype (A and B) , chymase-positive mast cell (MCC) phenotype (C , D) , and tryptase-chymase double-positive mast cell (MCTC) phenotype (E and F). Journal of Allergy and Clinical Immunology  , DOI: ( /mai ) Copyright © 2000 Mosby, Inc. Terms and Conditions

7 Fig. 6 Numbers (in cells per square millimeter) of eosinophils (A and B ) and IgE-positive cells (C and D ) in the epithelium (A and C ) and lamina propria (B and D ) in nasal mucosa biopsy specimens. Nasal mucosa biopsy specimens were taken before provocation, 1 hour after provocation, 24 hours after provocation, and 1 week after provocation. Allergen challenge (arrows) was performed with 1000 and 10,000 BU grass pollen. Box plots represent the 25th-75th percentile, the range (whiskers) , and the median value. Journal of Allergy and Clinical Immunology  , DOI: ( /mai ) Copyright © 2000 Mosby, Inc. Terms and Conditions

8 Fig. 7 The inflammation of mast cells, eosinophils, and basophils related to the total inflammation of these cells in the lamina propria over time. Journal of Allergy and Clinical Immunology  , DOI: ( /mai ) Copyright © 2000 Mosby, Inc. Terms and Conditions

9 Fig. 8 Tryptase-positive basophils. Double staining showed tryptase (red) and BB1 (blue) double-positive cells (counterstained methyl-green; magnification ×1000). Double staining with CD18, tryptase, and BB1 indicates that BB1-positive cells are positive for tryptase. Journal of Allergy and Clinical Immunology  , DOI: ( /mai ) Copyright © 2000 Mosby, Inc. Terms and Conditions

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