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Volume 115, Issue 2, Pages (August 1998)

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1 Volume 115, Issue 2, Pages 340-347 (August 1998)
The gastric H+,K+-ATPase is a major autoantigen in chronic Helicobacter pylori gastritis with body mucosa atrophy  Dirk Claeys*, Gerhard Faller‡, Ben J. Appelmelk§, Riccardo Negrini∥, Thomas Kirchner‡  Gastroenterology  Volume 115, Issue 2, Pages (August 1998) DOI: /S (98) Copyright © 1998 American Gastroenterological Association Terms and Conditions

2 Fig. 1 Immunohistochemistry of the gastric body mucosa. Autoantibodies reacting with canalicular structures within human parietal cells of the gastric body mucosa (original magnification 100×). No definite distinction between presence or absence of anti–H+,K+-ATPase reactivity could be made from the immunohistochemical staining pattern. Gastroenterology  , DOI: ( /S (98) ) Copyright © 1998 American Gastroenterological Association Terms and Conditions

3 Fig. 2 Reactivity of sera from 4 patients with autoimmune gastritis with the gastric H+,K+-ATPase. Sera were analyzed by immunoprecipitation (left) under nondenaturing conditions of the αβ heterodimeric H+,K+-ATPase, or its β subunit alone, expressed in oocytes and by Western blotting (right) on human gastric membranes under reducing (+βME, to detect the α subunit) and nonreducing (−βME, to detect the fully glycosylated β subunit) conditions.13 The immunoprecipitated H+,K+-ATPase is resolved by SDS-PAGE as a 94-kilodalton α subunit, a 50-kilodalton endoplasmic reticulum core glycosylated (βCG) β subunit, and a 60–85-kilodalton Golgi terminally glycosylated (βTG) β subunit. The high-molecular-weight bands are aggregated products of the highly hydrophobic α subunit. The serum from a neonatally thymectomized (nTx) mouse served as a positive control in immunoblotting.15 AIG, autoimmune gastritis; PA, pernicious anemia; βME, β-mercaptoethanol. Gastroenterology  , DOI: ( /S (98) ) Copyright © 1998 American Gastroenterological Association Terms and Conditions

4 Fig. 3 Reactivity of sera from H. pylori–infected patients with gastric H+,K+-ATPase. The sera from 9 H. pylori–infected patients and 1 patient with autoimmune gastritis and pernicious anemia (no. 6) were tested by immunoprecipitation (upper two panels) using oocytes expressing either the αβ heterodimeric H+,K+-ATPase or β subunit alone and by immunoblotting (lower panel) on human gastric membranes as described in Figure 2. Note that the endogenous α subunit (94 kilodaltons) of the Xenopus Na+,K+-ATPase coimmunoprecipitates with the β H+,K+-ATPase subunit, when expressed alone in oocytes, and becomes visible after long exposure of the PAGE gel.28 Gastroenterology  , DOI: ( /S (98) ) Copyright © 1998 American Gastroenterological Association Terms and Conditions


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