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Human cells produce type I and III IFNs upon Af stimulation.
Human cells produce type I and III IFNs upon Af stimulation. (A) Representative FACS plot of IFNLR1 expression in gated neutrophils or lymphocytes in human peripheral blood cells without treatment or 6 hours after culture with Af. (B and C) IFNLR1 expression as measured by MFI in each gated population in human peripheral blood (B) or bone marrow (C) samples. (D and E) qRT-PCR of relative gene expression of IFN genes and type III IFNR in RNA isolated from human peripheral blood (E) or bone marrow (D) samples. Data are means ± SEM of five (B and E) or four (C and D) individual donors. **P < 0.01, calculated by paired t test using Prism software. Vanessa Espinosa et al. Sci. Immunol. 2017;2:eaan5357 Copyright © 2017 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works
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