1. Efficient CRISPR mutagenesis in C. glabrata.
Efficient CRISPR mutagenesis in C. glabrata. Strain BG2 was transformed with pV1326 (top left), pV1329 (top middle), pV1329 with a stop-codon repair template (top right), pV1435 (bottom middle), or pV1435 with a stop-codon repair template (bottom right). The yellow box (bottom left) presents a magnified view of a pV1329 with stop-codon repair transformation plate (top right). Similar results were seen with strain CLIB138. Vectors depict CaCas9 promoter differences between the two plasmids.
Valmik K. Vyas et al. mSphere 2018; doi: /mSphere