1. Fig. 8. Zbtb7a deficiency impairs antigen presentation by AMs.
Zbtb7a deficiency impairs antigen presentation by AMs. Adoptively transferred Zbtb7a-sufficient or Zbtb7a-deficient huCD68-Kd Tg AMs in Fig. 6 were antigen-pulsed (with L-OVA or L-BSA delivering 5 μg of protein or 1 μg of OVA peptide) in vivo at 15 weeks after transfer, harvested at 6 hours after pulsing, and transferred (1 × 105) with 5 × 106 CFSE-labeled naïve OT-II T cells into C57BL/6 recipients. (A) Representative plot of OT-II T cell proliferation in lungs and spleen measured by CFSE dilution at 72 hours after AM and T cell transfer. Cells were pregated for CFSE+ and CD3+. n = 5. SSC-H, side scatter height. (B) Summary of the CFSE dilution. Mean ± SEM is plotted for T cell proliferation in the lungs. n = 5.
Deepak K. Nayak et al., Sci Transl Med 2017;9:eaal1243
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