1. FACT correlates with active gene expression.
FACT correlates with active gene expression. (A) qPCR of Ssrp1 levels among stem (mESCs), cancer (N2a and B16), and differentiated cell lines (MEFs and NIH3T3). Mean and SE of three biological replicates are shown. Data were normalised to Gapdh mRNA levels. (B) Heatmap assessing the mRNA levels (RPKM) of FACT (Ssrp1 and Supt16) at different time points of differentiation of ES cells to cardiomyocytes (Wamstad et al, 2012). (C) Distribution of SSRP1 relative to the TSS (±2,000 bp) and the TES (±2,000 bp) for four different gene classes ranked by the level of RNA abundance (high, medium, low, and very low). (D) Same as (C) but for SUPT16. (E) Scatterplot of the log2 SSRP1 coverage versus log2 mRNA expression. ESC, embryonic stem cells; MES, mesodermal cells; CP, cardiac precursors; and CM, cardiomyocytes.
Constantine Mylonas, and Peter Tessarz LSA 2018;1:e
© 2018 Tessarz and Mylonas