1. Radio-HPLC analysis of the biosynthetic product (after chemical N-benzoylation; Rt = 39.6 ± 0.1 min) generated by the recombinant O-phenylpropanoyltransferase by using [13-3H]baccatin III and β-phenylalanoyl-CoA as cosubstrates.
Radio-HPLC analysis of the biosynthetic product (after chemical N-benzoylation; Rt = 39.6 ± 0.1 min) generated by the recombinant O-phenylpropanoyltransferase by using [13-3H]baccatin III and β-phenylalanoyl-CoA as cosubstrates. The upper trace shows the radioactivity profile (in millivolts) and the lower trace shows the absorbance profile (A254) of authentic (3′RS)-2′-deoxytaxol. The radioactivity peak at 21.3 min is residual substrate.
Kevin Walker et al. PNAS 2002;99:20:
©2002 by National Academy of Sciences