1. Stable interactors of Plk1 include phosphorylation-dependent interactions.
Stable interactors of Plk1 include phosphorylation-dependent interactions. (A) Diagram depicting the distribution of kinase inhibitor–sensitive (yellow) and kinase inhibitor–insensitive (blue) Plk1 interactors and, below, their relative enrichment in candidate PBD motifs [Ser-Ser-Pro (SSP)/Ser-Thr-Pro (STP); dark gray] or not (light gray). (B) Experimental design to identify kinase activity–dependent Plk1 interactors using wild-type and Pincer mutant Plk1. HeLa cells expressing with Myc- or Flag-tagged wild-type or Pincer mutant Plk1 were arrested in mitosis and treated with MG132. Plk1 and its interacting proteins were immunoprecipitated, resolved by gel electrophoresis, and analyzed by LC-MS/MS. (C) Diagram depicting the distribution of kinase inhibitor–sensitive (yellow) and kinase inhibitor–insensitive (blue) Plk1 interactors and their ability to bind (white) or not bind (black) to Pincer mutant Plk1.
Arminja N. Kettenbach et al., Sci. Signal. 2018;11:eaaq1441
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