1. Bile acids regulate the ontogenic expression of ileal bile acid binding protein in the rat via the farnesoid X receptor 
Sandy T. Hwang, Nancy L. Urizar, David D. Moore, Susan J. Henning 
Gastroenterology 
Volume 122, Issue 5, Pages (May 2002)
DOI: /gast
Copyright © 2002 American Gastroenterological Association Terms and Conditions

2. Fig. 1
BA gavage induces IBABP mRNA expression in day 16 rat ileum. (A) Representative Northern blot of ileal total RNA isolated from day 16 rats who received the following treatment on days 13–15: untreated (C), vehicle gavage (V), dexamethasone (D), taurocholate gavage (BA), and dexamethasone and taurocholate gavage (D/BA). The blot was probed for both IBABP and β-actin mRNA. S, pooled adult standard. (B) Quantitative data from Northern blot analysis. IBABP mRNA was normalized to β-actin mRNA and then expressed as a percentage of the adult standard. Values are given as mean ± SEM (n = 3 for C group, n = 5–6 for the remaining groups).
Gastroenterology  , DOI: ( /gast )
Copyright © 2002 American Gastroenterological Association Terms and Conditions

3. Fig. 2
BA gavage increases IBABP protein expression in day 16 rat ileum. (A) Typical Western blot of total ileal homogenates from same groups of animals as in Figure 1. The blot was incubated with rabbit polyclonal anti-IBABP sera and subsequently with goat anti-rabbit antibody conjugated to horseradish peroxidase. (B) Quantitative data from Western blot analysis. IBABP is expressed as a percentage of the adult standard. Values are given as mean ± SEM (n = 3 for the C group, n = 5–6 for the remaining groups).
Gastroenterology  , DOI: ( /gast )
Copyright © 2002 American Gastroenterological Association Terms and Conditions

4. Fig. 3
FXR mRNA is present at all ages in the rat ileum. Typical Northern blot of total ileal RNA from untreated rats at different postnatal ages. The blot was probed with FXR cDNA and then reprobed with β-actin cDNA. Numbers represent the age in days. S, pooled adult standard.
Gastroenterology  , DOI: ( /gast )
Copyright © 2002 American Gastroenterological Association Terms and Conditions

5. Fig. 4
Specific IR-1 binding activity in nuclear extracts from adult rat ileum. Either FXR/RXRα proteins (lanes 1–5) or nuclear extracts (10 μg) from an adult rat ileum (lanes 6–10) were incubated with the radiolabeled IR-1 probe in an electrophoretic mobility gelshift assay. Competition analysis was performed with 100- or 1000-fold molar excess of unlabeled IR-1 probe (cold IR-1) or an IR-1 probe with mutations in its hexamer sequence (cold IR-1m).
Gastroenterology  , DOI: ( /gast )
Copyright © 2002 American Gastroenterological Association Terms and Conditions

6. Fig. 5
Supershift assay identifies FXR in nuclear extracts from adult rat ileum. Nuclear extracts from adult rat ileum (10 μg) were incubated with the radiolabeled IR-1 probe in an electrophoretic mobility gelshift assay. Supershift assays were done with anti-FXR antibody or a nonspecific antibody (mouse anti-STAT5).
Gastroenterology  , DOI: ( /gast )
Copyright © 2002 American Gastroenterological Association Terms and Conditions

7. Fig. 6
Lack of FXR binding activity in nuclear extracts from day 12 rat ileum. Nuclear extracts from rat ileum (10 μg) at different ages were incubated with the radiolabeled IR-1 probe in a representative electrophoretic mobility gelshift assay. Numbers represent the age in days with triplicate animals shown at each age.
Gastroenterology  , DOI: ( /gast )
Copyright © 2002 American Gastroenterological Association Terms and Conditions

8. Fig. 7
FXR and RXRα protein levels increase in the rat ileum during the third postnatal week. Western blots of total distal ileal homogenates from untreated animals at different postnatal ages. The blots were incubated with goat polyclonal FXR antibody (upper panel), rabbit polyclonal RXRα antibody (lower panel), as well as mouse monoclonal β-actin antibody. Numbers represent ages in postnatal days (n = 2–3 at each age).
Gastroenterology  , DOI: ( /gast )
Copyright © 2002 American Gastroenterological Association Terms and Conditions