1. Inhibition of pressure natriuresis in mice lacking the AT2 receptor
Volkmar Gross, Wolf-Hagen Schunck, Horst Honeck, Anna Franca Milia, Eva Kärgel, Thomas Walther, Michael Bader, Tadashi Inagami, Wolfgang Schneider, Friedrich C. Luft, M.D. 
Kidney International 
Volume 57, Issue 1, Pages (January 2000)
DOI: /j x
Copyright © 2000 International Society of Nephrology Terms and Conditions

2. Figure 1
Relationship between renal perfusion pressure (RPP) and urine flow (UV, A) and sodium excretion (UNaV, B) in AT2 receptor knockout (N = 8; •) and wild-type (N = 8; ▪) mice. *P < 0.05, values compared at equivalent RPP levels. The pressure diuresis and pressure natriuresis relationships for AT2 receptor knockout mice were shifted rightward.
Kidney International  , DOI: ( /j x)
Copyright © 2000 International Society of Nephrology Terms and Conditions

3. Figure 2
Relationship between renal perfusion pressure (RPP) and renal blood flow (RBF, A) and renal vascular resistance (RVR, B) in AT2 receptor knockout (N = 6; •) and wild-type (N = 8; ▪) mice. *P < 0.05, values compared at equivalent RPP levels. The RBF was decreased in AT2 receptor knockout compared with wild-type mice as RPP was increased. AT2 receptor knockout mice had a greater RVR compared with wild-type mice.
Kidney International  , DOI: ( /j x)
Copyright © 2000 International Society of Nephrology Terms and Conditions

4. Figure 3
Relationship between renal perfusion pressure (RPP) and cortical (A) and medullary (B) blood flow in AT2 receptor knockout (•) and wild-type (▪) mice. *P < 0.05, values compared at equivalent RPP levels. Cortical blood flow was well autoregulated in AT2 receptor knockout (N = 8) and wild-type (N = 10) mice. Step-by-step increases in RPP increased medullary flow signals in wild-type mice (N = 6) but had no influence on medullary blood flow in AT2 receptor knockout mice (N = 7).
Kidney International  , DOI: ( /j x)
Copyright © 2000 International Society of Nephrology Terms and Conditions

5. Figure 4
Relationship between renal perfusion pressure (RPP) and glomerular filtration rate (GFR, A), fractional sodium excretion (FENa, B), and fractional water excretion (FEH2O, C) in AT2 receptor knockout (•) and wild-type mice. *P < 0.05, values compared at equivalent RPP levels. (Columns in A) GFRs of all pressure levels for wild-type (▪) or AT2 receptor knockout mice () are summarized. GFR was not different between AT2 receptor knockout (N = 8) and wild-type (N = 7) mice. Fractional sodium (Na) and water (H2O) excretion curves of AT2 receptor knockout mice (N = 8) were shifted toward the right compared with wild-type mice (N = 7).
Kidney International  , DOI: ( /j x)
Copyright © 2000 International Society of Nephrology Terms and Conditions

6. Figure 4
Relationship between renal perfusion pressure (RPP) and glomerular filtration rate (GFR, A), fractional sodium excretion (FENa, B), and fractional water excretion (FEH2O, C) in AT2 receptor knockout (•) and wild-type mice. *P < 0.05, values compared at equivalent RPP levels. (Columns in A) GFRs of all pressure levels for wild-type (▪) or AT2 receptor knockout mice () are summarized. GFR was not different between AT2 receptor knockout (N = 8) and wild-type (N = 7) mice. Fractional sodium (Na) and water (H2O) excretion curves of AT2 receptor knockout mice (N = 8) were shifted toward the right compared with wild-type mice (N = 7).
Kidney International  , DOI: ( /j x)
Copyright © 2000 International Society of Nephrology Terms and Conditions

7. Figure 5
Expression of renal AT1-receptor mRNA in AT2-receptor–deficient and wild-type mice. (A) RNase-protection assay from renal RNA showing AT1 receptor (352 bp) versus reduced glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (83 bp) expression. Y+ = cDNA-probes incubated with yeast RNA and RNase. Y- = cDNA-probes incubated with yeast RNA and without RNase. (B) Quantitation of AT1-receptor mRNA expression after autoradiographic signal analysis. Data are normalized to GAPDH-mRNA expression (N = 6). *P < Expression of renal AT1-receptor mRNA was higher in AT2 receptor knockout mice than in wild-type mice.
Kidney International  , DOI: ( /j x)
Copyright © 2000 International Society of Nephrology Terms and Conditions

8. Figure 6
Expression of cytochrome P450 mRNAs in the kidneys of wild-type and AT2 receptor knockout mice analyzed by means of RT-PCR (discussed in the Methods section). The RT-PCR products obtained starting from the poly(A)RNAs of four different wild-type mice were loaded on lanes 1 through 4 and those of four different AT2 receptor knockout mice on lanes 5 through 8. Note that systemic differences were not detectable between AT2 receptor knockout and wild-type mice.
Kidney International  , DOI: ( /j x)
Copyright © 2000 International Society of Nephrology Terms and Conditions