1. Development of human T-cell subsets in NSG hL-7xhIL-15 humanized mice.
Development of human T-cell subsets in NSG hL-7xhIL-15 humanized mice. (A) Representative flow cytometry plots of thymic T-cell subset of conventional NSG and hIL-7xhIL-15 KI NSG (IL7xIL15) humanized mice. (B) Frequencies of thymic CD4+ T cells, CD8+ T cells, and CD4+CD8+ T cells in NSG (n = 17) and hIL-7xhIL-15 KI NSG humanized mice (n = 12). (C) Representative flow cytometry plots of splenic T-cell subsets in a hIL-7xhIL-15 KI NSG humanized mouse. (D) Frequencies of splenic CD4+ T cells, CD8+ T cells, CD45RA− memory CD4+ T cells, and CD45RA− memory CD8+ T cells in conventional NSG (n = 7) and hIL-7xhIL-15 KI NSG (n = 5) humanized mice. (E) Representative flow cytometry plots showing a lack of TCR Vα24+Vβ11+ NKT cells in the BM, spleen, and thymus of a hIL-7xhIL-15 KI NSG humanized mouse. Error bars represent mean ± SEM (*P < 0.001, by two-tailed t test).
Masashi Matsuda et al. LSA 2019;2:e
© 2019 Matsuda et al.