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Missing MS2 transition My trouble is, when the PRM raw file was imported, most of transition was not extracted, but all precursors were extracted, even.

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Presentation on theme: "Missing MS2 transition My trouble is, when the PRM raw file was imported, most of transition was not extracted, but all precursors were extracted, even."— Presentation transcript:

1 Missing MS2 transition My trouble is, when the PRM raw file was imported, most of transition was not extracted, but all precursors were extracted, even if the library was built from the same raw file and nice MS2 spectrum have been displayed.

2 Before import raw file Example 1 After import raw file

3 Before import raw file Example 2 After import raw file

4 A few of good extracted peak as below:

5 The following is my data processing steps.

6 Step 1 Peptide setting

7 Build library

8 Step 2 Transition setting

9 Step 3 Import results


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