1. Volume 123, Issue 5, Pages 1485-1493 (November 2002)
Evolution of hepatitis C viral quasispecies after liver transplantation 
Andre C. Lyra, *, Xiaofeng Fan, *, Dorothy M. Lang, ‡, Karina Yusim, ‡, Sanjay Ramrakhiani, *, Elizabeth M. Brunt, §, Bette Korber, ‡, Alan S. Perelson, ‡, Adrian M. Di Bisceglie, * 
Gastroenterology 
Volume 123, Issue 5, Pages (November 2002)
DOI: /gast
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2. Fig. 1
RNA levels before and after OLT in 4 patients with mild and 7 patients with severe recurrent hepatitis C. RNA was measured between 12 and 80 months after OLT (median, 14 months) in (A) the mild group, and between 3.4 and 80 months in (B) the severe group (median, 27 months).
Gastroenterology  , DOI: ( /gast )
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3. Fig. 2
Intrasample HVR1 diversity of all samples included in the study. Patients 1–4 had mild recurrent hepatitis C, whereas patients 5–11 had severe recurrent hepatitis C. The x-axis represents serum and/or histologic follow-up in months of each individual patient; thus the scale is different in each graph. Negative numbers represent time before transplant. The y-axis represents the intrasample diversity at each time point. The stage of fibrosis is also noted at each time point. The closed circle and open triangle symbols correspond to intrasample genetic distance and nonsynonymous substitutions per nonsynonymous site, respectively.
Gastroenterology  , DOI: ( /gast )
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4. Fig. 3
Intrasample HVR1 diversity. (A and B) Mild group. Comparison between mean group values from before (analysis of 4 samples, 40 clones) and from all time points after OLT (8 samples, 80 clones) showed a significant increase (P = for genetic distance, P = for nonsynonymous substitutions, and P = for synonymous substitutions in the HVR1). In the flanking regions, these same values were P = for genetic distance, P = for nonsynonymous substitutions, and P = for synonymous substitutions. (C and D) Severe group. Comparison between before (7 samples, 70 clones) and after (11 samples, 110 clones) OLT showed P = for genetic distance, P = for nonsynonymous substitutions, and P = for synonymous substitutions in the HVR1 and P = for genetic distance, P = for nonsynonymous substitutions, and P = for synonymous substitutions in the flanking regions.
Gastroenterology  , DOI: ( /gast )
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5. Fig. 4
Correlation of amino acid complexity and stage of fibrosis after OLT. All pairs of serum samples and liver histology (n = 19) obtained at the same time from all 11 patients were analyzed (r = −0.543; P = 0.016). Samples after retransplantation were not included in the analysis.
Gastroenterology  , DOI: ( /gast )
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6. Fig. 5
Overall intersample diversity/year between time point 0 and time points after OLT from the mild (8 time points) and severe (11 time points) groups. (A) HVR1. Genetic distance was 131 × 10−3 and 105 × 10−3 (P = 0.016), nonsynonymous substitutions per nonsynonymous site were 124 × 10−3 and 132 × 10−3 (P = 0.503), and synonymous substitutions were 146 × 10−3 and 37 × 10−3 (P < 0.001) in the mild and severe groups, respectively, for each comparison. (B) Flanking regions. Genetic distance was 31 × 10−3 and 21 × 10−3 (P < 0.001), nonsynonymous substitutions per nonsynonymous site were 12 × 10− and 9 × 10−3 (P = 0.002), and synonymous substitutions were 82 × 10−3 and 64 × 10−3 (P = 0.002) in the mild and severe groups, respectively, for each comparison.
Gastroenterology  , DOI: ( /gast )
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7. Fig. 6
Neighbor-joining trees of 462-bp nucletide sequences including HVR1. Trees were constructed with the distance matrix calculated using the Kimura 2-parameter method in the MEGA program. (A) Tree of patient 4 from the mild group. The circle, triangle, and square symbols correspond, respectively, to samples from 15 months before OLT (control), time point 0, and 12 months after OLT. (B) Tree of patient 6 from the severe group. The circle, triangle, and square symbols correspond, respectively, to samples from 22 months before OLT (control), at time point 0, and from 12 months after OLT.
Gastroenterology  , DOI: ( /gast )
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8. Fig. 7
Rate of amino acid change over time from all patients in HVR1 and flanking regions. The x-axis represents time of follow-up in months; negative numbers represent time before OLT. The y-axis represents the average amino acid change. The rate of amino acid change is most variable in the first 3 years after OLT and appears to become constant after 36 months.
Gastroenterology  , DOI: ( /gast )
Copyright © 2002 American Gastroenterological Association Terms and Conditions