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Creatine Kinase Activity in Human Spermatozoa and Seminal Plasma Lacks Predictive Value for Male Fertility in In Vitro Fertilization 1  Claus Rolf, Hermann.

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Presentation on theme: "Creatine Kinase Activity in Human Spermatozoa and Seminal Plasma Lacks Predictive Value for Male Fertility in In Vitro Fertilization 1  Claus Rolf, Hermann."— Presentation transcript:

1 Creatine Kinase Activity in Human Spermatozoa and Seminal Plasma Lacks Predictive Value for Male Fertility in In Vitro Fertilization 1  Claus Rolf, Hermann M. Behre, Trevor G. Cooper, Birgit Koppers, Eberhard Nieschlag  Fertility and Sterility  Volume 69, Issue 4, Pages (April 1998) DOI: /S (97)

2 Fig. 1 Electrophoretic separation of ejaculates prepared according to Huszar et al. [5]. The shaded areas indicate the location of the creatine kinase (CK) isoenzyme peaks of the patient’s samples. The open areas indicate the location of the creatine kinase isoenzyme peaks of the commercial standard containing CK-MM, CK-MB, and CK-BB. Top panel: Creatine kinase activity in the washed pellet of an azoospermic patient. Major peak CK-BB. Total creatine kinase activity = 133 U/L. Bottom panel: Creatine kinase activity in the washed pellet of a normospermic patient. Major peak CK-Mi Mi. Total creatine kinase activity = 284 U/L. Fertility and Sterility  , DOI: ( /S (97) )

3 Fig. 2 Creatine kinase (CK) activity in seminal plasma from fertile and infertile men. Lower and upper margins of the boxes indicate 25th and 75th percentiles, horizontal bars on the whiskers mark 10th and 90th percentiles. In addition, all values are plotted individually. The median is indicated by a line within the box. Fertility and Sterility  , DOI: ( /S (97) )

4 Fig. 3 Total creatine kinase (CK) activity in washed spermatozoa from fertile and infertile men (A) and distribution of creatine kinase isoenzymes (CK-Mi/creatine kinase total ratio) in these groups (B). Lower and upper margins of the boxes indicate 25th and 75th percentiles, and horizontal bars on the whiskers mark 10th and 90th percentiles. In addition, all values are plotted individually. The median is indicated by a line within the box. Fertility and Sterility  , DOI: ( /S (97) )

5 Fig. 4 Total creatine kinase (CK) activity in swim-up purified spermatozoa from patients with different IVF outcome (A) and the specific distribution of creatine kinase isoenzymes (CK-Mi/creatine kinase total ratio) in these groups (B). Lower and upper margins of the boxes indicate 25th and 75th percentiles, and horizontal bars on the whiskers mark 10th and 90th percentiles. In addition, all values are plotted individually. The median is indicated by a line within the box. Fertility and Sterility  , DOI: ( /S (97) )


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