1. False-color Raman images of spleen tissue sections from control and LPS-treated animals obtained by k-means cluster analysis.
False-color Raman images of spleen tissue sections from control and LPS-treated animals obtained by k-means cluster analysis. (A) Representative Raman false-color images of spleen tissue sections from control mice (four animals, mouse A, B, C, and D) and LPS-treated mice (four animals, mouse E, F, G, and H) obtained by k-means cluster analysis. The x-axis and y-axis show step size (3 μm) used for Raman imaging. The spectra were processed to remove cosmic spikes and background corrected using SNIP algorithm. Raman fingerprint region (600 cm-1 to 1800 cm-1) has been used for analysis. The k-means algorithm was executed to find ten different clusters of spectra from control and LPS-treated animals. The contribution of each cluster as cluster scores has been represented on right side of the individual tissue sections as cluster scores. (B) Corresponding Raman spectra of the clusters obtained from k-means analysis using the same color code as in (A). Spectra of clusters 1, 4, 5, 8, 9, and 10 are shown, whereas k-means cluster spectra 2, 3, 6, and 7 are omitted, as they correspond to individual image pixels with high fluorescence background that were registered on the spectrometer detector.
Anuradha Ramoji et al. ImmunoHorizons 2019;3:45-60
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