1. Investice do rozvoje vzdělávání
Micromanipulation (Metody pro mikrosopickou manipulaci s nanokonstrukty)
Zbyněk Heger
Investice do rozvoje vzdělávání

2. Micromanipulation
Very small structures, concurrently enlarged via microscope.
Commercially used for assisted fertilization.
Many possibilities in nano-, micro-scale research. 2

3. Assisted fertilization
In vitro fertilization (IVF).
Louise Joy Brown – 1. IVF born by IVF ( ).
Hormonal induction of superovulation (E2 levels).
Oocytes picking up
Fertilization (intracytoplasmic injection of sperm – ICSI – low sperm motility, small number of oocytes, immunological infertility)
Embryo transfer 3

4. Devices Inverted microscope with programmable micromanipulators,
Microinjector,
Microelectrode,
Piezoelectric microdrill,
Software. 4

5. Patch clamp
Neher and Sakmann - the Nobel Prize in Physiology or Medicine in 1991
Microelectrophysiology
´´Patch microelectrodes´´
Current of single ion channels – connection in
fundamental cell processes
Spheroplasts
Patch clamp record 5

6. 6

7. Micromanipulation
Maimon et al., Nature Neuroscience 7

8. Micromanipulation
Semyanov et al., Nature 8

9. Micromanipulation
Sridharan et al., Journal of Neural engineering 9

10. Micromanipulation
Pavenstadt et al., American Journal of Physiology 10

11. Investice do rozvoje vzdělávání
Acknowledgements
Investice do rozvoje vzdělávání 11

12. Thank you for your attention