1. Gas chromatography Software

2. TRACE GC System Components
1- Pneumatic Compartment
The pneumatic compartment contains
the pneumatic gas control circuits.
The circuits can be completely electronic (digital pneumatics), completely analog,
or a combination of analog and digital components (mixed pneumatics).
2- Analytical Unit
The analytical unit consists of two sub compartments:
the column oven
the injector and detector compartment

3. Cont. 3- Electronic Compartment
The electronic compartment consists of two sub compartments:
the high-voltage compartment
the motherboard for the detector control cards
4- Display and Keypad
The display and the keypad make up the TRACE GC user interface.

4. Sentinel Key
The Sentinel Key is delivered with Chrom-Card software and it should be connected to the parallel port of your computer as follows:
Remove the printer cable from the PC parallel port LPT1.
Connect the Sentinel Key instead the printer cable to the PC parallel port LPT1.
Re-connect the printer cable on the other end of the Sentinel Key.

5. Start Chrom-Card
click twice on the icon of the relevant instrument.

6. Chrom-card main menu

8. New Method Parameters Method Title Detection Parameters
Integration Parameters.
Calculation Parameters
Report Parameters
Report Stripchart
Operator ID
Analytical Information

9. 2. Detection Parameters Start Acquisition when GC starts
The start of acquisition is achieved by hardware contact between the instrument
and PC. The automatic start will have effect only if this option has been enabled.
Baseline compensation
Through this function you can select the blank chromatogram to be subtracted to all chromatogram analyses.

10. 3. Integration Parameters
This dialog box contains all parameters used for the integration algorithm.
The integration algorithm is very simple and similar to the one used in a stand alone integrator.

11. 4. Calculation Parameters
This section is the analytical method part containing all the parameters for peak calculation.
Calculation
This box defines the calculation method used to convert peak areas into concentrations.
Calibration

12. 5. Report Parameters
The Report Format box permits the selection of various Report types providing various options

13. 6. Report Stripchart
It permits to select and to set the Stripchart options to be included in the report printout.

14. Standard Report
A standard Report can be made consisting of all integrated peak information. The
detailed report consists of:
Component names
Calculated concentrations or area %
Retention times
Areas
Baseline correction
Response factors
Relative retention time if working with relative retention time identification
The report column BC identifies the baseline correction of the peak.
The available codes are:
RS = Resolved FU = Fused
TL = Tailing CR = Carried
MI = Manually integrated ^^ = Off-Scale peak

15. 7. Operator ID / 8. Analytical Information

16. TRACE GC Parameters
The TRACE GC (oven, carrier, injectors and detectors) should be set according to your GC configuration.

17. Oven Parameters ( Trace )
The Oven tab provides you full control of the GC oven including sub ambient options and post run programs.
Controls are for running complex temperature programs (seven levels of ramps) and controlling post run events.
The Oven tab displays several control groups: Graph, Ramps, Post Run Conditions,
Acquisition Time, and Oven.
The graph displays a graphical representation of the oven temperature program including any post run events.
An isothermal run just gives a flat line.

18. Analytical Method
An analytical method describes the treatment a sample receives during analysis. You can set parameters for:
temperatures, including seven programmed changes (ramps)
Pressures
flow rates
inlet types
detector types and parameters
signal changes
autosampler parameters
timed events before, during, and after the run

19. Injector Parameters
Injectors parameters page control the Split/ Splitless injector.
Depending on your instrument configuration the window displays the appropriate fields for that inlet.
For example, if you selected a right SSL inlet this tab displays.

20. Carrier Parameters
The carrier gas flows through the GC column at a specific rate, measured either as a linear velocity (cm/sec) or as a flow rate (cc/min).
Common carrier gases are Helium, Hydrogen, and Nitrogen.
Hydrogen offers the best chromatographic properties (optimum resolution at the highest flow rates).
However, because Hydrogen is flammable, Helium is often used as an alternative for a carrier gas.

21. Detector Parameters
Detectors monitor the composition of the column effluent.
This is accomplished by measuring some physical or chemical property of the eluted compounds.
FID, ECD, NPD,FPD, PID, TCD and mass are typical detectors for a GC.
The detector types that show up in the TRACE instrument setup window are determined by your selections in the Instrument configuration window.

22. Detection parameters
The detection parameters module is the analytical method section that contains all
the parameters for data acquisition.

23. Auto sampler Parameters

24. Internal Standard Parameters

25. Components Table
The Component table is the analytical method section that contains all the parameters for the peak identification.
The Component table offers a number of tools to enter the retention times and identification windows through the chromatogram .

26. Fill Components Table
fill the Component table in an automatic way, using the retention time of the peaks calculated from the chromatogram currently displayed.

27. Sample table
The Sample Table is the analytical method section that contains all the information about the sequence of samples to be acquired and processed.

28. Concentration table First
The user should address the standard component of interest by clicking the mouse on the concentration table grid.
Second
The cursor will automatically address the input box, ready to accept the new value, once the new value has been entered and confirmed by pressing the (17(5 key, the system updates the concentration grid.
Alternatively
You can address the box by a double click, in this case the system permits to edit the previous content of the box

29. Fill Samples Table
Fill the sample table in an automatic way, the user supplies common information for a batch of samples and the system will automatically fill the lines with the sample name and the filename made by a root name and an increasing index.

30. Sample Type Sample amount
SA is the amount of sample material measured.
This column is only available for Unk samples.
Internal Standard
IS is the amount of internal standard added to the analysis samples.
Units of measurement must be the same as those used in measuring the sample amount.
Dilution Factor
The XF is a scaling factor (multiplier) which may be used as a conversion factor.
This column is only available for Unk samples

31. Sample being Acquired
The system permits to view the chromatogram being acquired with the function View Sample Being Acquired.

32. View Calibration Curve
According to the calculation method, the system shows the calibration points with the peak area and the concentration.

33. View Chromatogram
View Chromatogram is a tool for Chrom-Card users to view or modify the peak integration.

34. Recalculation
The Recalculation tag permits to recalculate sample batches. Chrom-Card 32 bit will perform the recalculation of the samples first-last (included) using the selected option.
Sample Sequence
You should supply the first-last sample to be reprocessed. Refer to Recalculate sample(s) from Sample Sequence Box.