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Published bySucianty Lanny Kusnadi Modified over 5 years ago
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C/EBPβ regulates FAM134B-2 and autophagy regulators in the mouse liver.
C/EBPβ regulates FAM134B-2 and autophagy regulators in the mouse liver. (A) Scheme of construct design for targeting the C/EBPβ transgene. (B) qRT-PCR analysis of FAM134B-2 in livers from WT and L-C/EBPβ tg mice under a fed state. (C) Immunoblot analysis of FAM134B-2, ATG5, Beclin-1, and LC3B in livers from WT and L-C/EBPβ tg mice under a fed state. *Non-specific signal. (D) Immunoblot analysis of FAM134B-2 in livers from WT and L-C/EBPβ tg mice treated with leupeptin (i.p., 15 mg/kg body weight) for 4 h. (E) Immunoblot analysis of FAM134B-2 in livers of L-C/EBPβ KO mice under starvation. Mice were fasted for 16 h. (F) Quantitative densitometry of the immunoblot analysis. *Nonspecific signal. Student’s unpaired t test was used for statistical analysis. *P < Source data are available for this figure. Shohei Kohno et al. LSA 2019;2:e © 2019 Kohno et al.
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