1. Overexpression of cardiac MED13 alters cardiac metabolism AExpression of fatty acid oxidation and Krebs cycle genes in MED13cTg heart determined by RNA deep sequencing, n = 3.
Overexpression of cardiac MED13 alters cardiac metabolism AExpression of fatty acid oxidation and Krebs cycle genes in MED13cTg heart determined by RNA deep sequencing, n = 3. B, CMED13cTg hearts have decreased (B) acylcarnitines and (C) long‐chain acyl‐CoA species in the fed state, as measured by metabolomics, n = 5. D–FIn conditions simulating the fed state, MED13cTg hearts displayed (D) normal coronary flow rates, (E) decreased oxygen consumption, and (F) decreased long‐chain fatty acid (LCFA) substrate oxidation rates, as determined by Langendorff heart perfusions and 13C NMR analysis, n = 4–6. G–IIn conditions simulating the fasted state, MED13cTg hearts displayed (G) normal coronary flow rates, (H) increased oxygen consumption, and (I) increased endogenous substrate oxidation, as determined by Langendorff heart perfusions and 13C NMR analysis. JIn conditions simulating the fed state, when glucose is readily available, the oxidation ratio shifts towards a preference for glucose in MED13cTg hearts. In conditions simulating the fasted state, when LCFAs are more readily available, oxidation ratios are similar in WT and MED13cTg hearts, n = 4–6. Data information: Data are mean ± SEM, t‐test.
Kedryn K Baskin et al. EMBO Mol Med. 2014;6:
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