1. Increased TLR7 transcripts in biallelic naive B cells.
Increased TLR7 transcripts in biallelic naive B cells. (A) Total TLR7 mRNA (raw Ct values in real-time PCR) plotted against the relative abundance (RA) of the less expressed allele in each cell according to the allele-of-origin assay. Each dot denotes one cell; n = 134 cells pooled from three female donors. Q1, low-expression range as in (B). Highest-likelihood monoallelic and biallelic strata are color-coded as orange (RA < 10%) and blue (RA > 35%), respectively. (B) Violin plots for the same experiment, total TLR7 expression normalized to the mean. The first-quartile (Q1) threshold splits cells into lower or higher expression groups; two-sided Fisher’s exact test for proportions in the monoallelic and biallelic strata. (C) Relative TLR7 transcript quantitation in the monoallelic and biallelic strata. Each dot represents one cell’s ΔCt with reference to TLR9 (difference of means, −0.567 Ct units); the TLR7-adjacent gene, TMSB4X (−0.534); MYD88 encoding a TLR-signaling factor (−0.797); and housekeeping gene B2M (−0.743); P values from Welch’s t test (TLR9, TMSB4X, and MYD88) or two-sided Mann-Whitney test (B2M). (D) Principal components analysis of the single-cell transcript quantitation data set for 30 immunity, chromosome X, and control genes, showing the individual cells and group centroids for the monoallelic and biallelic strata.
Mélanie Souyris et al. Sci. Immunol. 2018;3:eaap8855
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