1. UNDERSTANDING THE CELL MEMBRANE
Single Cell Egg Lab Day 2
UNDERSTANDING
THE CELL MEMBRANE

2. Success Criteria Learning Targets
Conduct an investigation to understand the structure and function of cell membranes
Conduct the Naked Egg Lab and record data

3. Overall Guidelines
The egg has soaked for 7 days in vinegar to dissolve the shell
You will be testing an assigned chemical on your egg.
The eggs are very fragile.
Handle the egg as little as possible. Use the cup to move it around (not your hand)
Wash your hands thoroughly when done handling the egg.

5. Which solution will have the greatest affect on an egg’s mass?
GROUP A Red Drink
GROUP B Water
GROUP C Ammonia
GROUP D Vinegar
GROUP E Soapy Water
GROUP F Run-off Water
GROUP G Salt Water
GROUP H Sugar Water
GROUP I Motor Oil
GROUP J Simple Green

6. IDENTIFY VARIABLES Treatments/liquids egg soaks in Size (mass) of egg
Amt of treatment egg is soaked in, length of time soaking

7. MAKE A HYPOTHESIS (Choose One Below)
The egg will lose mass
The egg will gain mass
The egg’s mass will stay the same

8. MATERIALS Deshelled egg Cup Gloves, Apron, Goggles Electronic Balance
Ruler
Sharpie
Assigned Liquid Solution in 2 liter bottle with lid
Tape/straw as needed

9. ACCURATE REPORTING Put your initials on the cup with the sharpie.
Add the period number and group letter
Add the date & time

10. PROCEDURES:
Obtain a cup from the teacher & measure it’s mass in grams using a balance.
Record the beginning cup measurement.
3. Place egg gently in the cup and measure it’s mass with the cup in grams using a balance.
Record the total mass of egg plus cup.
Subtract step 2 from step 4 and record (This is the egg’s beginning mass).
Report the mass to your teacher and verify it is correct.
Gently shake the assigned solution by rocking it back and forth in the sealed bottle.
Quickly remove the cap and pour it over the top of the egg until completely covered.
Use a sharpie to mark the beginning height of the egg.
If floating only: Use a straw with tap to hold down the egg.
Place the egg in the assigned location
Wait for the assigned amount of time directed by your teacher
Record observations and drawings (Make sure the data table is correct)

11. Units? Make before you put it in treatment:
What does the egg look like? Feel Like?
Do this before you add treatment…

12. Other ways to use an Electronic Balance
Turn on, push the zero button.
Place container on the balance. Hit RE- ZERO. This automatically subtracts the weight of the container.
Add egg, record mass.
Remove both container & egg.

13. 24-48 hours later
Locate your egg & record qualitative observations in your data table.
Draw a image of your egg
Record the date & time of measurement.
Record how many hours the egg was in solution.
Use a sharpie to record the egg’s new height.
Use a ruler in millimeters to measure the difference and record on the data table.
Carefully, pour remaining liquid into the sink without pouring out your egg. (Use the spoon to keep the egg from leaving the cup).
Measure the mass of the egg in the cup.
Subtract the original mass of the cup to determine the remaining mass of the egg.
After Completing data collection, return the egg to the teacher.
The class will POP the eggs together to inspect the inside
Prepare to make qualitative observations of the opened egg.
Calculate the percentage of change to determine what percent of the eggs new size represents how much it changed? (Follow math instructions on data table)
Report the percentage of change to the teacher for the class data table