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Changes in the cooling rate and medium improve the vascular function in cryopreserved porcine femoral arteries  Montserrat Rigol, PhD, Magda Heras, MD,

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Presentation on theme: "Changes in the cooling rate and medium improve the vascular function in cryopreserved porcine femoral arteries  Montserrat Rigol, PhD, Magda Heras, MD,"— Presentation transcript:

1 Changes in the cooling rate and medium improve the vascular function in cryopreserved porcine femoral arteries  Montserrat Rigol, PhD, Magda Heras, MD, Anna Martínez, MD, María Jesús Zurbano, BS, Elba Agustí, BS, Eulàlia Roig, MD, José Luís Pomar, MD, Ginés Sanz, MD  Journal of Vascular Surgery  Volume 31, Issue 5, Pages (May 2000) DOI: /mva Copyright © 2000 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

2 Fig. 1 Dose-response curve to norepinephrine in rings preparation of femoral arteries (n = 3 arteries; 12 rings). Values are expressed in milligrams. Journal of Vascular Surgery  , DOI: ( /mva ) Copyright © 2000 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

3 Fig. 2 Relaxations in response to increasing concentrations of acetylcholine in fresh and cryopreserved arteries stored for 1, 15, 60, and 180 days. Values are expressed as percentage of the induced contraction by 10–7 mol/L norepinephrine. Journal of Vascular Surgery  , DOI: ( /mva ) Copyright © 2000 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

4 Fig. 3 Light micrographs obtained from cross-sections of everted segments of fresh (A) and cryopreserved arteries stored for 60 (B) and 180 days (C). The arrows show endothelial cells. In all cases, the interaction of platelets with the endothelium is almost absent. (Toluidine blue stain; original magnification, ×850.) Journal of Vascular Surgery  , DOI: ( /mva ) Copyright © 2000 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

5 Fig. 4 Scanning electron micrographs of fresh (A) and cryopreserved arteries stored for 15 days (B). In both cases, the endothelium is confluent, and the cell morphologic condition is normal. (Original magnification, ×200.) Journal of Vascular Surgery  , DOI: ( /mva ) Copyright © 2000 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

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