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Differential intracellular localization of ISS in human PDCs
Differential intracellular localization of ISS in human PDCs. Purified PDCs were cultured with fluorescent ISS for 90 min, then fixed and stained intracellularly with antitransferrin receptor (TfR) or anti–LAMP-1 antibodies and imaged by confocal microscopy as described in Fig. 4. Differential intracellular localization of ISS in human PDCs. Purified PDCs were cultured with fluorescent ISS for 90 min, then fixed and stained intracellularly with antitransferrin receptor (TfR) or anti–LAMP-1 antibodies and imaged by confocal microscopy as described in Fig. 4. Between 25 and 75 cells from at least five different donors were analyzed for colocalization between the ODN and either transferrin receptor (TfR) or LAMP-1 (LP1). Cristiana Guiducci et al. J Exp Med 2006;203: © 2006 Rockefeller University Press
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