1. Cellular localization of r-proteins during repression of a 40S or a 60S r-protein gene.
Cellular localization of r-proteins during repression of a 40S or a 60S r-protein gene. Pgal-eS31 or Pgal-eL43, each carrying plasmid-borne genes for uS5-GFP or uL23-GFP expressed from their native promoters, were grown in synthetic galactose medium and shifted to synthetic glucose medium for 16 h. The cells were inspected by confocal microscopy. A uniform distribution of the GFP-tagged protein between the nucleus and cytoplasm indicates that the export of ribosomal precursor particles carrying the tagged protein is normal. A buildup of the GFP-tagged in the nucleus indicates that ribosomal precursor particles carrying the tagged protein are not matured to nuclear export competency.
Brian Gregory et al. LSA 2019;2:e
© 2019 Gregory et al.